FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34436
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTCAGCGAGCACTCAGACGTACACATGTATATCGCTAGACGTGTGTGTCTGTCTCTTATACACATCTCCGA860.24973864560343825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG150.00233612469.039
AGGTAGC150.00233612469.01
CACATGT150.00233612469.026
CGGTTGG208.3975494E-569.041
GTGTGTC150.00233612469.047
AGACGTG150.00233612469.041
AGACGTA150.00233612469.019
CGCTAGA150.00233612469.037
TCGCTAG150.00233612469.036
ACATGTA150.00233612469.027
ACGTCAG150.00233612469.03
TAAGGAA150.00233612469.061
GTTATGG150.00233612469.01
AAGGGGG501.4551915E-1162.10000269
GGTGAAA308.940093E-657.557
GGGTGAA252.532231E-455.256
GTACACA200.007298824451.7523
CCAGGGG200.007298824451.7526
GAATCTC609.094947E-1151.7535
TATATCG200.007298824451.7532
Produced by FastQC (version 0.11.2)