FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55580
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGTT208.443194E-569.059
AATGTCG150.002343735769.036
CGGCAAA252.5459105E-455.251
GGCTTGT252.5459105E-455.249
CCATCCC252.5459105E-455.24
TCGCGTA252.5459105E-455.239
CTTGTGG200.007322420451.75000451
TTTAGTG200.007322420451.7500047
TGGTTGA200.007322420451.75000455
AGTGCGT200.007322420451.75000417
GGGGTTG200.007322420451.75000440
CTCGGTT200.007322420451.75000439
CTCGGAT200.007322420451.75000439
GGGAAAA200.007322420451.75000459
GAAGTTA200.007322420451.75000460
CTCATCG200.007322420451.7500047
TTACGTG200.007322420451.75000444
CAGCTAC200.007322420451.75000448
TTTTGCC200.007322420451.7500048
CGCGTAT200.007322420451.75000440
Produced by FastQC (version 0.11.2)