FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112923
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG3200.024.79687569
CGATCTT52050.021.4755065
GATCTTT37050.019.740896
AATCGCG1402.0836195E-417.24999836
GATCTGG27250.017.0917436
CGATCTG89450.016.8546685
ATCTTTT39300.016.679397
GGACCGT6250.016.5600016
GGCGAAT11050.016.54751268
CCGATCT234450.016.4222664
GTATTAA7500.016.11
CGAATCT3006.8030204E-1016.134
TTCCGAT241900.015.8594462
TCCGATC246750.015.6316123
ACGGCCG5600.015.40178520
ACCGTTA6350.015.2125988
TAGGACC9100.015.1648354
CTTCCGA255500.015.1502941
CGCCGTT5700.015.13157925
GATCTTG16150.014.953566
Produced by FastQC (version 0.11.2)