FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31491
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG800.2540408370645581TruSeq Adapter, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGTG208.386331E-569.035
AGGGGGG150.00233425169.069
GTCTTAC150.00233425169.01
TCTGCTT301.09621396E-769.053
AGGAATG1000.062.10000233
GGAATCT1400.061.60714334
AATCTCG750.059.80000336
GTGTAAA353.184814E-759.14285758
AGGAATC2300.058.50000433
GGAATGT308.922267E-657.534
CTCGTAT601.8189894E-1257.539
CGTGAAA308.922267E-657.557
GAATCTG601.8189894E-1257.535
TCTCGTA601.8189894E-1257.538
ATCGGGG308.922267E-657.537
GGGGATT252.5288685E-455.240
GGGTGTA252.5288685E-455.256
AAATCAA252.5288685E-455.267
GGTGTAA252.5288685E-455.257
TGGCGTG252.5288685E-455.245
Produced by FastQC (version 0.11.2)