FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972210
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT48950.022.0606045
GATCTTT37400.018.9107196
TAGCGCC1200.001477538517.2499759
CGATCTG95600.016.8172045
TTGTACG1452.7239206E-416.6554284
ATAGCGC1503.5275207E-416.0999768
GATCTGG30500.015.9494266
CCGATCT255050.015.9212394
TTATACT12700.015.7561484
GTCCGCT3101.0841177E-915.58062250
AGACGTA62600.015.54151169
TTCCGAT264450.015.4596822
GCCGCTT1350.003244691515.3333150
TCCGATC271750.015.0951723
TATAGCG1605.761206E-415.0939822
ACTGATC17050.014.9735858
ATCTTTT45000.014.8733127
CTTCCGA279300.014.7118261
GTATATA21600.014.694671
GTATTAG10600.014.6464521
Produced by FastQC (version 0.11.2)