FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042675
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1202.4593937E-623.06
GATCTTT24300.020.7283956
ATACGAC1258.745262E-519.326
CGATCTT32650.019.2312415
TTGTCCG900.005735910519.16666628
ACGGGTC1501.6949669E-518.40000266
TCTATAC6600.018.2954543
GTATTAG5500.018.1909081
TAGTACC3400.017.2500024
TTTCGGA2151.2763558E-616.0465131
TGCGCGT1756.360479E-515.77142945
ATCTTTT30000.015.7557
ATAGTAC5350.015.4766343
GCGTAGG2252.0496009E-615.3333341
CGCTAGG1350.003244196315.33333259
CGGGTCG2051.4438565E-515.14634267
AGACCGT1400.004132298814.7857146
GTATTAT8200.014.7256111
GTCGGAG3756.730261E-1114.7260
ATAATAC9850.014.710663
Produced by FastQC (version 0.11.2)