FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931389
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCG1251.2551027E-1030.35993248
CGCGTCC1409.348954E-619.71424147
GTCCGGT1651.8297087E-618.8181450
GACGCGT1301.1815748E-418.57688168
CGATCTT34350.018.179475
AGGCGTT2307.2741386E-917.9999669
CGCCCTA1150.001110612417.9999658
GCGGTCG1200.00147729217.24996267
TGCGCGT1602.952086E-517.2499645
GTATTAG5500.016.9367641
GCGCGTC1855.450902E-616.78374546
TCGCCGC1653.843848E-516.72723657
AGTCGTT1452.7236663E-416.65513469
TACACTG8800.016.4662975
GATCTTT24650.016.2356786
CGATCTG69900.016.1398665
CGGTCGG2804.7366484E-916.0178268
GTATAAG6400.015.6331821
TATGCCG1554.5261803E-415.5806143
AGGGCGA3552.910383E-1115.54926263
Produced by FastQC (version 0.11.2)