FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480525
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1004.336815E-420.69995933
CGCCGTT1851.1779775E-820.5134725
CGATCTT42700.018.7446925
CGATCTG75650.018.7435195
TACGGCC2255.5424607E-918.39996119
GTATAAG7800.018.1345771
GATCTGG25800.018.0526526
CGATCGT2208.5830834E-817.24996634
CGGTACG1200.001477451117.24996438
GGACCGT3106.002665E-1116.6938516
GCACGAA2702.924935E-916.61107643
GCGGTAC1250.001941492516.55996537
ACGGCCG2552.6024281E-816.23526220
GATCTTT34300.016.0935846
TACGTCG1300.002522511215.9233666
CGATCGA1300.002522850415.92304453
CCGATCT207350.015.9064054
TAGACGT11300.015.8760744
TTCCGAT214000.015.5572112
CCGCCGT2452.9216608E-715.48976424
Produced by FastQC (version 0.11.2)