FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327379
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG2900.020.2244914
CGATCTT38550.018.7046075
ATACGAC3600.017.2503013
AACGCGG5800.017.24993121
TAATACT7700.017.0262724
CGATCTG80150.016.9597475
GTATAGA7600.016.7963451
CGACGGT3501.8189894E-1216.7577956
GATCTGG27250.016.5859666
GAACGCG6050.016.53712320
GTACGCC3157.6397555E-1116.42850569
ATTGCGA3401.4551915E-1116.2352318
ACCGTTA4050.016.1854678
ACGGTGC4700.016.1492188
GATCTTT27500.016.0588056
GCGAACT6750.015.84437927
GTATTAT7500.015.6402731
AGTTCCG3101.0841177E-915.58058232
TTGCGAC3552.910383E-1115.549232519
CGCGTTC2001.1430049E-515.52493958
Produced by FastQC (version 0.11.2)