FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length75
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2416.666666666666664No Hit
GAAAAGCAGTACAGTGAGCAACCCCAGACCCATCACAGTTTATACAGAACATCACCAATACTTTTTAGCTCTTGT10.6944444444444444No Hit
CCTCAAATTGATCCAAATATTCATTTGCATATTCTTCAAAAAGCCAATTTCCAAACTGCTCAATCACAAGAAACT10.6944444444444444No Hit
CCGAAGACTCTTACATGATCCAGAAATAAACTTCTATTGTGTTTGTGCCATTAAACATGTATCAGTCTATATGTC10.6944444444444444No Hit
ATCATGTATCGTGAATGTTACCTATTATCGTAGCTATCTCCATCCCTATCTAGAGCCATCTTTTTAAAATGGTGT10.6944444444444444No Hit
CCACTGCACCCTCTCAGCCAGGGCTGCGACAGTGCCGGGCTGGGAGCAAAGCACTTCATAAATGCAGAAGGATGT10.6944444444444444No Hit
TCCCTGCCGTGAGCTCACTCGTGTCGGGGAGATGGCAGTTTAGGAGCGTGGGGCAGGAGTGTTGAAGATTGAGTA10.6944444444444444No Hit
GTTTATATGAGTCCTTATGTGTTAGGTGAGTCTCCTGAAGGCAGCAGATAGTTCGTTGCTGAGTTTTTATTGATT10.6944444444444444No Hit
CCTCAAAGATTTTACATAATATCGCGCAAAAATCTGTATGAAATCTAATATTGATTTATAGAATGATAGAGACCA10.6944444444444444No Hit
GACAAAGGCCTCATACGGTAGTACTGTATTTAACGCGGTAAGGGAGAAGGAGTCCAGGCTAAGAATCGTCGTATA10.6944444444444444No Hit
CTAACGGGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAACCTCGGAGTGGGAAGGAGA10.6944444444444444No Hit
CCACATCTCTAGCGCGAGGCCCAGGGCCCGTTGGCTCGTGGCCCCCGGGAAAGCCACAGTGGAGTGGCGGTGTAG10.6944444444444444No Hit
CCAGAGTTTGAAAAATGACGCTTCCATTCTGGTGCCTCACAAAGATCTAACATGTCAGCGAATTACATCCTCTCT10.6944444444444444No Hit
GCCATTTAAGATGACTATTTGAATTTCGTTTTTCTTGTTAAATGATTGTGAGGCCTACTCAGCGATTTTGAACTA10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAATAACAAAAAAA10.6944444444444444No Hit
GCCAGCAGTCGAGCCTCAACTTGGCAGCTACTACCACTCAGCCTCCTGCCTGGCTGCTTCAAGTCTGGTGGTAAA10.6944444444444444No Hit
ATACATGAGTCTCAAAAGTGTCAACTGAAAAGGTAAATGATAACAAGGAAGCATATACCAAAATGTTTACATGGA10.6944444444444444No Hit
GTGATACACTAAGGAATATCAATATCATCAAATTGGTATCTTCCCAAAAATGTAGCTTGATTCTAATCATGAGAA10.6944444444444444No Hit
ATAATTTTTGGTTGCACTTCACACTGTAACAAGGAAATATTCCCACTGCCAAAAAACATTTGAGAAAGAAGCAGT10.6944444444444444No Hit
ATCATTTAGTTACTGGCTTTAAGCAAAATAATTATGATGAGCTTTGGTATATGCTGTTTGTGTCTGTATCTGTGT10.6944444444444444No Hit
GAGCTAACTGCATCCTGTGGACTCAGAATTTTGAGGACTCAGAAAGCAAATCACATTCCCTAGTGCTGTGCACTT10.6944444444444444No Hit
CTGAAAAACAGAGCAGCAAACAAAATACGCAGAATCCAAACATAATACAGCTTCATAAGAAAAAAAAAAAAAATT10.6944444444444444No Hit
GAGCACACGTCTCCCAGTGCAGATCCTCACAGATAACGTCTCCGACTGGAGAACGAGACGGATAGCAAGTCCCAG10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAA10.6944444444444444No Hit
AAGCAGACGGGTAATCTGAGGTCAGGAGTTTGAGACTACCCTGACGAAGGGAGAAACCCCGTCTATACCAAAAAA10.6944444444444444No Hit
GTGTATAAAGGGTCCAAGAAGTCAAGGTGCCACTGGGGTCACATTTCTGTCTCTATCAGATTAAATTACAGCTCT10.6944444444444444No Hit
AAACTAGACAGAAGCATTCGGAGAAACTTCTTTTTAGTGTGTGCATTCATCTCACAGTGTTGAAACTTTCTTTTG10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAA10.6944444444444444No Hit
AACCACACTTCTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGAAAGCGAAAAAAAAACCTAAAAAAAAAA10.6944444444444444No Hit
ATATAAGACAGAACATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAAAACCACACAAGAACAGAATGAA10.6944444444444444No Hit
GTCTGGCAGCGCTGAACGGAAGAGCACACGTCTGCCTCTCGATTAGGCAAGTGCTCGGGACTGCCTGCGGGGAAG10.6944444444444444Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
ATATTGGTCAGGCTGGTCTCAAACTCCTGACCTCAGGTTAGCCTCCCACCTCAGCCTCCCAAAGGGATTACAGGC10.6944444444444444No Hit
ACACATCTCCGACCCCACCAGACTCCTGAGCAGCTCGGAAGTCGTAATATGGAAGATAATAAAAAATCGGGGCCG10.6944444444444444No Hit
CGTCTCCCTCAGGAGATCGGAAGAGCACACGTCTCCCTCTGGAGATCGGAAGAGCACACGTCTCCCTCTGGAGAT10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAA10.6944444444444444No Hit
TCCTTGAAGACCGGCAGGCTTGTGGTAAAAAGCAACGTGCCGGGGAGCTTGTCATAAAGACTGAGCAAAAATGGG10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATCCAAAAAAGTTAAGATTTTAAACTTCACCCCTCCACACCAACCTGC10.6944444444444444No Hit
AGTGAAAACATGCATTAATCATTTCAAAGAATCCTGTGGCTACGCAAAAAGTCATTGAGAGAGGGAAAGCTGATT10.6944444444444444No Hit
TAAAAATTACATTGAGGGTTCCTCTAATCCCAGCTAATCATGATCCTGAGGCAGGAGAAACCATAGAACCCAGCA10.6944444444444444No Hit
GCCATAGGCACATCCTGAGAGATACAGGTTCCATTATTTGCCTGTATGGAACTGAAGCCTCTGGAATTCATGAAC10.6944444444444444No Hit
ATATACACCAATGTGTTATATATCAAAGACAACTGGATAAAAGGCTGGCAGTGTTAGTTTCCTTTTCAAAAATAA10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATAGAAAAT10.6944444444444444No Hit
AAACAAGGCCCGCCCAAAGCATTAATCTCTGCCATAATTCGGTCACGTGCCCATCCTTCAGGCAAAGCATCGATA10.6944444444444444No Hit
CTGTTTACTGTCCTTGGCTGAGGGAATTGAGGCTCGGAGTTTACTGTTAAGAGAACGCTTATTGTGACTGATTGC10.6944444444444444No Hit
CAAACTAAAACATCGGAATAAAACACTAGAATCAAGACCCTCAGGTTAAAATTATTCCTCTTCAAAAAAAAATAT10.6944444444444444No Hit
AAACAGAACACGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAACACAACCAAATATAAAAAAAA10.6944444444444444No Hit
GTTATAAAACATGATCTCTAAGGAGACGACTAATAGGAGGCTTAAAATAATTATCATTTTACAATTATAAAATTT10.6944444444444444No Hit
AATATTTTTTTTTTTTTTTTTTTTTTTTTTTATATATAATAATAAAAACCGCTATACATATGTTTATGAACAAAG10.6944444444444444No Hit
CTGTTCCGGGGGCTCTTCCGATCTATAGTGGGGTGTGCTCTTCGGAAAACTGTCTCTTATACACATCTCCGAGCC10.6944444444444444No Hit
GTTATACATGTTATTTGATGTCTGTTTCAAATAACTGTCAGCTACACTTTCCTGAAGTAGCAACATTTCTGTGAA10.6944444444444444No Hit
AAGATGACCTTCACATGCCAATTCCAATTCCCAGAAGTCTTCTCCCTTTGGGCTGTCTCTTATACACATCTCCGA10.6944444444444444No Hit
CCCCATTTTTGAGGACAAGGATGGCCCAGTTTTATCTGACACTCTGTATTCGAAAAGTGCATTAAGATTGCAAAC10.6944444444444444No Hit
GGACGAAATAGCTGACTTCAAAAATTTTGATGTGATGTCCTATCGACTATAATGCTGGAAGATTGTACTATTCGA10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6944444444444444No Hit
CGCTTAAATATCAAATTAGTAATTTAAACAATAAGTAAGATCTGAGGAAAGATTACATCCAAGTTTTTTGAAGCT10.6944444444444444No Hit
AAAATAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATTCAAAAAACAGACACCACACCAA10.6944444444444444No Hit
CAAAAGTAAATAAACAAGCAATAAGAAGCAACCCAAAAAATAACAGAGCAAAAAGACCCAATGAGATGCTGAATT10.6944444444444444No Hit
GCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATCTAGCCATTGCACTCCAGCCTGGGCTATGAGAGAGC10.6944444444444444No Hit
GATCTGCTCCGGGCCTGGCCTGCTGTCCCTCCCACACTTGCCATCAGCTTAGTTTGCCTTTCAGGACCTAGCTGG10.6944444444444444No Hit
CTCCCTGGGGAGATCGGAAGAGCCCACGTCTCCGTCTGGTGAGGGGTAGAGCACCCGTCTCGGTGGGGAGAGGGG10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTATGACAGAGTACAATGGACTAGGTGGCCTAAAGAACATTTATTTCTCATGATTCTGAAGGCTGGAAATTCCA10.6944444444444444No Hit
CACCTGGGCATGTGGGTGCTGTTCCAGTCTGTCACATGCTCACCTGGGGGTGTGGGTTCTGTTCCAGGCGGTCGC10.6944444444444444No Hit
GGAAAAAGATGTCTGTCAGAATAGTCAACTCGAGCTTGGGTTCATATTCAGAAAGTCATACATCTAGGCGAGATA10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAA10.6944444444444444No Hit
GAGTACTAACCGTCCAGAGCCACCAGGAGCACCTTCGGGTGCCGGGTGTGGTGATGCACGGGACCTTTCCTGGTA10.6944444444444444No Hit
CATAAAAATAGAGCATCATGTGATCACTATTTGTCTGGAAGCACACATGCTATCATTTCACTAGTTGGTGGGAGA10.6944444444444444No Hit
CCCACCACAGATCGGAAGAGCACACGACTCCCAGGGGAGATCGGAAGAGCACACGTCTCCCAGGGGAGATCGGAA10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGTTACAGACTACACTCCTTGACTATGATGGGAAGGTACCAGCTGTTTTGAAGAAGAAACCAAAGCAACCATTT10.6944444444444444No Hit
AGCATTGTCTTAGACCAAAACGTGGCCAGCTGCGGAGCCATTCAGCCGGACGTCATGCACTTTGATGCCTGCCTC10.6944444444444444No Hit
GGAATTCGAAGGGTGCTAGCGTTAATCGGAATTACTGGGCGTAACGCGCGCGTAGGTGGTTTGGTAAGATGGATG10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATA10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAACTCCTGGACTCAACCGATATTAAAAACAAAGCAACCCAAAAACAACA10.6944444444444444No Hit
ATCTAGAACAAGATGACATAGAAAGGTTCCAAAGAAAAACTCAGAAAACAACAGTAACCCCCAATTAAAAAATGG10.6944444444444444No Hit
AGTATGAACTTACTCAAAATTTACCTATCTAAAATATGAAATCATAAACAAGGGAAAGTATTAGAATCATTTATA10.6944444444444444No Hit
GATCAACAGAGTGAGTCTCTGAAAATACATAATAAAAAATTTTGGCCCGGGCTTCGAAGTGAATGAATTTAGACT10.6944444444444444No Hit
CTACAAGCAAAACATGAAAAGCCAGTATAATAAAGGATTTAGAGAACAATATCAAAACTGATAACAGCAGTATAC10.6944444444444444No Hit
TAAATAAAAAGTACTAATTAAAATATCTCCTGTTTCTACCAAAAAGTCAACATTTTCAACACTAAAAGGAAATAA10.6944444444444444No Hit
TAAGAGCCCAACTCTCACATTGAACATACGAATGTATAAAATACGCATATAAGCGCACACCATACTAATTTGTCT10.6944444444444444No Hit
GGGAGGGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGGGGGGGGTGAGAAAAA10.6944444444444444No Hit
ACCCTTCGAAGATCGGAAGAGCACACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAACAGGACGAA10.6944444444444444No Hit
GAGCACTTCCCAAAAAAATCTCTGAATACAATAACCATCTCAGCGTCCGTATGCTCAGAAGCCCATCTAAGGCAC10.6944444444444444No Hit
CACCACTTACGATATCCCAAGAGCCCACAATCCACCAGCGCAGCTCATATTACCTCAGCGGGGGGATAAAAAAGA10.6944444444444444No Hit
GAGTGATATATAGCCTTTATGCCGTATCAATATATCTTGACAATACTTTAATTCAAAGTGAGTTAATGGGTTCTA10.6944444444444444No Hit
GCTTACCTCTCGCATCGGTCGGTACCGTATCTTCATAATCATTGGCCCGATTGTCGCGGCGGGTGGCATGGTGCT10.6944444444444444No Hit
GTGCTAGGATGACAGGCATGAGGCATTGCTCCTGGCCTGTGCAGGGTTTTTCCAGCTAAGGAAAGTTTCCCCTGC10.6944444444444444No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGCTGAGACAAAAGCAACAGA10.6944444444444444No Hit
ACACTCAACACATCGAAAGAGAACACGAAACTACTTATAATCAAAATTGACTAAAGTGTGCCAATAAAATAAAAA10.6944444444444444No Hit
GAGGAAGAGCCCCACAGAAGATCGGAAGAGCACACGAGACTCCTGAGCATCTCGTTTTGGCTGGTATGCTTGGAA10.6944444444444444No Hit
CCCGTAAGAGATCGGAAGAGCACACGTCTCCCAGGAGAGATCGGAAGCTGTCTCGTCTACCAGTCTACGACCGCA10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATATATTTGCTCTGACAAGCCATAGCGAGCTACTCACTTCACAGCAATTTATAGCATTTCCTCGACAATTTCAAT10.6944444444444444No Hit
CTTCCGATCTATGCAGGGAGACGTGTGCGCTTCCGAGCTATCTGTCTTATACACATCTCCGAGCCCACCAGACGA10.6944444444444444No Hit
GTTGTAATACTATCTTATATTTTTATATAATAATATATTAAATGTTTATAAATGCCAAAAATCCCCAACTTTTAA10.6944444444444444No Hit
AACATAGACACAAAGTAACAGAAAAACACAAATAAATATAAATATAATACAGTCTTAATATTGAAAAAAAAAAAA10.6944444444444444No Hit
AAACTGACCAACATGGAGAAACTCCATCTCTGTCTCTTATACACAAATCCGAGAACAAGAGACGGCAACGAATAG10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGTTAAACAGAGTTAACA10.6944444444444444No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAGCAGGGGATAACGCCATGGCCAGATGATGGCCCACACGGCCAAAAA10.6944444444444444No Hit
CACTACACACAGACCCACATAGAAACACATACATAAACACAGTCACACACACACACACACCCATGCATGACACAG10.6944444444444444No Hit
GGCTTAAAGACTTATATGGAAGACCTATATCTATAAATACCTTAGAAGGAAACCTATAATATCCCACTCTGGACA10.6944444444444444No Hit
GTTTTGCTATTGTTGACCAGGCTGGAGTGCAATTGTTAGACTGTCTCTTATACACATCTCCTAGACGCAGAGACG10.6944444444444444No Hit
CTCAGATAGCTGACTTCAGAGAGAGAGCAGGTTGTAAATTCTTTCTTATCAGACCTAAAAGGGTGCCTGGCTCTT10.6944444444444444No Hit
CATCCAAGTTTAAAATTATCAGCGAAATGGTCCATGTTTTTCCAATTACCTGCTGACACGGTTCTAATCTAAGTG10.6944444444444444No Hit
ACCCTCGACATAACAAAAAAGAACACGATTCCCATTGAGCATCCTTAGAGAACTCCAAATGACTCTTCATGAGAA10.6944444444444444No Hit
CCCAATGAGAGATAGGAATAGACCAAGATGCAAAAAAGCATCTTGGATTGAATAATGAAAATATAAAAAACAAGA10.6944444444444444No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6944444444444444No Hit
CTATACTGTAGGGAATAATGACAGGGAAAAAAGTCTGTACATGTTCAGTAGAGCTGCAATATTTTTCCCAAATAT10.6944444444444444No Hit
CCCCCGCAAAGATCGGACGAGCCCACGAGACTACTGAGACTAGAGGGTACGGGCAGTCGGGTGCAAAAAAAAAAG10.6944444444444444No Hit
GATAAAATTCATCTTACTGAAGACATGGTCGCTTATCATATTTTATCTTAAAACAATATAAACAAATATAACTTA10.6944444444444444No Hit
AATAGATATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTCTCAGCACCACATTGCACTTATTA10.6944444444444444No Hit
CCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCTACTGATCGAAGCCTT10.6944444444444444No Hit
AGAGCACACCCGGTCCAGATCGGAAGAGCCCACGAGACTCCTGAGCATCGGGTATGCCCTCTTCGGCTGGGAAAA10.6944444444444444No Hit
AAAGATTGTGAGAAAAGCCTATTTAAAATTTACTTAGGTTATTTGAACCTAAAGCTGTCCTCTACATTAGCAAAC10.6944444444444444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGCAAACCACAA10.6944444444444444No Hit
GAACCAAAGCTCACAATAATAAGAGAGCACATAGCAAGATTAAAAATCACCAAAAGATAAAATTGACCCAAAAAA10.6944444444444444No Hit
ATCAAAATCTGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCACCAGCCCCTCATACAAT10.6944444444444444No Hit
CGTCTCCCTGGTTAGATCGGAAGAGCACACGTCTCCCGGGATAGATCGGAAGAGCACACGTCTCCCTGGTTCTGT10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAAGAGGACTGTTAAAAAATCAAGAATGAATTCACTATTTCTAAAAAAATATTTACCAGAATGTAAAACCCAGTA10.6944444444444444No Hit
GAAGAGCACCCGTCTCCCTACCTAGATCGGAAGAGCACACGTCTACCAACCAAGATCGGAAGAGCACACGTCGTG10.6944444444444444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTTTATTGTTATTTTGAAATTCACATGAGATTACTTCCAGAGGAAATATCGGTTTGCTGCCTTTCTTTGAAGGC10.6944444444444444No Hit
CCTACAAATTGAGCTGCTGGCCTTGCATTTAATCATTCAATAATATGGAGACTAATTATCATTTAGTGGTGTAAA10.6944444444444444No Hit
ATACTATACGAGAACGAACTTTCTGTAGCGCAGATGGGTAAATGCACTTATTCTCAGCCTGTTGCTGATCAACAT10.6944444444444444No Hit
CACATATTAATTCACCAGCAAAGTATGGGAGACACCCACAAACAAACCTGTCTCTTATACAGAACTCCGCGATCA10.6944444444444444No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers