FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225787
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG5000.22144764756163995RNA PCR Primer, Index 33 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG150.002353089269.0000141
CGGTTTA200.007351416651.75000422
TCGTCTA200.007351416651.7500049
CGTATGC3500.047.31428541
CCGTCTT2750.046.41818247
TCGTATG3600.046.040
CTCGTAT3550.045.67605639
TATGCCG3650.045.36986543
GCCGTCT2750.045.16363546
AGGGGGG1150.045.00000469
TCTCGTA3550.044.70422738
GTATGCC3900.044.2307742
AATCTCG4200.041.8928636
ATGCCGT3650.041.58904344
GGTAAAC350.001345520439.4285743
CGTCTTC2900.039.2586248
GCTTGAA3350.039.13432756
ATCTCGT4350.038.8620737
TGCCGTC3900.038.03846445
CAATCTC4950.036.24242435
Produced by FastQC (version 0.11.2)