FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31471
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3301.048584411045089RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG1000.31775285183184515RNA PCR Primer, Index 33 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG370.11756855517778272RNA PCR Primer, Index 33 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG360.11439102665946428RNA PCR Primer, Index 15 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG350.11121349814114583RNA PCR Primer, Index 33 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG208.386331E-569.041
ATCAGAT150.00233425169.042
CCGTGTT150.00233425169.047
GCCGGGG150.00233425169.046
TGGTTGA150.00233425169.055
GTATGGC253.024992E-669.042
CTCGGAT150.00233425169.039
TCTCGGA150.00233425169.038
GGTATCA150.00233425169.010
CGTGTTG150.00233425169.048
AGGGGGG353.9890438E-969.069
GCGTATG301.09621396E-769.040
TCGCGTA301.09621396E-769.038
AATCGCG301.09621396E-769.036
CGCGTAT353.184814E-759.14285739
GCGTGTT252.5288685E-455.247
TCTGCGT200.007293018551.7554
GTTGAAA200.007293018551.7557
CCGGGGT200.007293018551.7547
TGACGAT200.007293018551.756
Produced by FastQC (version 0.11.2)