FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945286
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4200.027.92852445
TATGCCG4550.025.78017643
CCGTCTT4900.024.64281547
ATGCCGT4950.024.39389844
TCGTATG5050.023.9108540
GCCGTCT5100.022.32349246
CGTATGC5400.021.08329641
GCGACGG2553.6379788E-1220.29408366
CTGACCG2051.7425918E-920.1954299
CGTAACG1056.026127E-419.71425253
CGATCTT50700.019.0532235
CGAACTA1653.8449354E-516.72724353
CGTCTTC7300.016.54106748
CGATCTG98000.016.4402775
CTCGTAT7000.016.26425739
GTATTAT10900.016.1421741
ATAATAC12500.016.0079733
CGACTCG1958.992041E-615.92304959
GTATAAT10000.015.8699721
GATCTTT39900.015.6506156
Produced by FastQC (version 0.11.2)