FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404957
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG3150.032.85713269
CGATCTT51050.018.9226195
GGCGAAT12100.018.24792968
GATCTTT35400.018.0296556
CGGTCGA1250.001941446716.55999463
CGATCTG91150.016.5403125
GATCTGG28700.016.4686376
TCTGTCG5300.015.6226378
TTTCGGA3552.910383E-1115.5496151
CCGATCT248950.015.4380364
GTATATA17100.015.1318891
ATCTCGC8700.015.0689627
CGCTCGA1851.0220533E-414.91891453
TTCCGAT258950.014.8018882
ATAGCGG1400.004132592614.78570926
TCGGGAC2101.8136341E-514.78570956
TACTGCG2101.8136341E-514.7857097
GCGAATC7500.014.719995569
CTAGACG8000.014.6624963
CTGTCGC4950.014.636369
Produced by FastQC (version 0.11.2)