FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395536
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1851.1779775E-820.5135146
TATGCCG1701.08613676E-720.29411943
ACGCTAG1056.025606E-419.71428543
GACCGTT1801.9835716E-719.1666667
CGATCTT34050.018.5418515
CGTTACG950.00783686918.15789216
ATCGACG950.00783686918.15789263
AATCGCG1150.001110701218.036
CGATCTG62850.017.3460625
TGCCGTC2005.995025E-717.2545
CCGTATG1402.083766E-417.2499989
CGCCGTT1855.451886E-616.78378525
CAATCGC1503.527123E-416.135
AGGACCG2804.7384674E-916.0178575
TATACTG9000.015.7166665
ACGGCCG2001.1429893E-515.52500120
ACGTCGC4700.015.41489354
CCGTTAT2503.6736856E-715.189
TGTCGCC4101.8189894E-1215.14634210
GTATTAA9150.015.0819681
Produced by FastQC (version 0.11.2)