FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915205
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG67350.037.4969255
TTCCGAT141800.037.1765672
CCGATCT141050.037.129654
TCCGATC145350.036.505933
CTTCCGA150550.034.9700241
CGATCTC54750.023.5672915
CGATCTA18050.023.5098635
AACGACG750.00199398222.9999318
GATCTGG45900.022.4740166
CGATCTT47550.022.202055
TCTTCCG76250.022.1254025
TATGCCG4500.021.46659943
ATCTGCG10450.020.4688367
ATCTCGC8400.020.1249377
CGCGTAA5350.019.99059121
GCGCTAG1651.8308965E-618.81812313
GCTCTTC89900.018.5741023
GTGCTCT89800.018.3258531
GATCTCG25750.017.2836176
GCGCGTA6400.017.24994720
Produced by FastQC (version 0.11.2)