FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278130
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCGA1951.8189894E-1224.7692321
GCGACGG2450.022.53061366
CGATCTT45550.021.3589485
CGACGGG2451.8189894E-1221.1224567
TGCCGTC2201.9099389E-1020.38636245
GTATCGC1056.025482E-419.7142851
ATCCGAT2406.0936145E-1018.6875022
GACGGAT950.00783675218.15789610
GATCTTT39850.018.0075286
CGATCTG69600.017.8448285
ATCTACG4450.017.8314597
CCGATCT193600.017.214364
TTCCGAT198250.016.7757872
TCCGATC203300.016.5796873
TATGCCG2301.4245234E-716.543
TCTACGC3401.4551915E-1116.2352928
CTTCCGA212350.015.8243461
AATCTCG3950.015.72151936
GATCTGG24650.015.5354966
GTATTAT10750.015.4046521
Produced by FastQC (version 0.11.2)