FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200765
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT181250.019.0917115
CGATCTG413450.017.606845
GATCTGG141150.016.1563226
CCGATCT1066850.015.4189514
TTCCGAT1095550.015.0559032
TCCGATC1128500.014.6988443
CTTCCGA1131050.014.6504531
GTATATA92300.014.5027481
TATGCCG10250.014.13657143
GCGACGG12500.014.07598666
TAGACGT45650.014.0570464
TGTCGCC17400.013.87934810
GTATAGG43500.013.7207271
CTAGACG35550.013.4894893
GATCTTT144850.013.4094766
GGCGACG27000.013.28887565
GTATTAT60100.013.2030311
GTATTAG45900.012.9281411
TATAATA74700.012.8393942
TATACTG57950.012.799915
Produced by FastQC (version 0.11.2)