FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43701
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4230.9679412370426306RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG1140.26086359579872315RNA PCR Primer, Index 33 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG600.13729662936774903RNA PCR Primer, Index 33 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG560.12814352074323243RNA PCR Primer, Index 15 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG540.12356696643097412RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG450.10297247202581175RNA PCR Primer, Index 33 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACA150.002340376469.021
GGGTGAA150.002340376469.056
TACTTCC150.002340376469.053
GTCTACG150.002340376469.02
TTATATC150.002340376469.052
CTCGGTT150.002340376469.039
GTACTTC150.002340376469.052
TCGTCAA150.002340376469.027
GTTGGGC150.002340376469.03
ATACCCC150.002340376469.015
TACACCA150.002340376469.046
TCGCGTA401.1603333E-860.37538
TTGTGCT252.5398715E-455.252
ATGGCGT252.5398715E-455.244
TATGGCG252.5398715E-455.243
TTATGCT252.5398715E-455.252
CAATCGC501.0732037E-955.235
TCTGGTT252.5398715E-455.253
TCTGGGT252.5398715E-455.253
GTGTTGT200.007312008651.7549
Produced by FastQC (version 0.11.2)