FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171692
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG7380.4298394799990681TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG5310.30927474780420755TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG208.4936044E-569.047
GAGGGGG309.093132E-657.569
AGGGGGG1850.052.21621769
GGTAAGA200.00734843151.7545
ACCTACG200.00734843151.7569
ATACAGC200.00734843151.7552
GTATGCC6650.048.2481242
TATGCCG6650.047.72932443
TCGTATG6250.047.47240
CTTGAAA6000.047.14999857
CGTATGC6600.046.52272841
CTCGTAT5200.045.77884739
TGCCGTC6750.044.9777845
GCCGTCT5850.044.82051546
TCTCGTA4400.044.6931838
TTGAAAA6650.044.61654358
CCGTCTT5850.044.2307747
CTGCTTG5600.043.74107454
ATGCCGT6950.043.68345344
GTCGTAT950.043.5789539
Produced by FastQC (version 0.11.2)