FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40450
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG6941.715698393077874TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG4491.1100123609394315TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG1300.32138442521631644TruSeq Adapter, Index 10 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG960.2373300370828183TruSeq Adapter, Index 10 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG780.19283065512978986RNA PCR Primer, Index 10 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG700.173053152039555TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG680.1681087762669963TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGGGGG630.1557478368355995TruSeq Adapter, Index 10 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG590.14585908529048208RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG480.11866501854140915TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG470.11619283065512978TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAT150.002339111969.000013
GTTCTAA150.002339111969.000012
GAGCTAA150.002339111969.0000151
TTATGCT150.002339111969.0000152
TCGCGTA150.002339111969.0000138
AGAGCTA150.002339111969.0000150
CCGTGTT208.41546E-569.047
GCCGTGT208.41546E-569.046
CGTCTTG252.5375988E-455.248
AGCTCGT252.5375988E-455.237
TGAGCTC252.5375988E-455.235
AGGGGGG950.054.47368669
TCGTATG3300.054.3636440
ATCTCGT2950.053.7966137
CTCGTAT3150.053.66666439
CGTATGC3550.053.45070641
TTGAAAA3500.052.2428658
GTATGCC3700.052.21621742
TATGCCG3650.051.986343
TCTGCGT200.00730808851.75000453
Produced by FastQC (version 0.11.2)