FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689325
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3550.032.07137340
CGTATGC4300.030.48870841
CGTACGC703.8531107E-529.57120558
TATGCCG4150.029.09616543
ATGCCGT4650.025.96754644
TGCCGTC4850.025.60805345
AGACCGC4000.025.0123169
CCGTCTT4700.024.95725847
ACCGTCC1401.3897079E-824.6426728
GCCGTCT5300.022.13190746
CGCGGGA2153.1523086E-919.25566713
CGATCTG70400.019.0144145
GTATGCC7000.018.7284342
CTCGTAT4850.018.49539239
GCGACGT1753.2106946E-617.74305366
ATCTGCG6150.017.3901147
CGATCTT43000.017.1699565
TCTCGTA4850.017.0726738
CGGTCAA1250.001941138616.5604935
CCGATCT197850.016.3216364
Produced by FastQC (version 0.11.2)