FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419107
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3350.029.86562545
GCCGTCT3400.028.41172446
TATGCCG3650.028.35612343
TCGTATG3700.027.97292940
CGTATGC4300.024.06973341
ATGCCGT4300.024.06973344
AGCGTAC800.00290037321.56246810
AGACCGC3850.021.50646269
GTATTAT8500.019.8886171
ATAGCGC1258.7462104E-519.3199718
TACGACG1301.18172466E-418.57689522
CTCGTAT4950.018.12118539
CCGTCTT5350.018.0560547
CGATCTT41750.017.8494455
TAATACT8750.017.7431964
TATACTG10000.016.9053235
AACCGCG1452.7240082E-416.65514832
AGTACCG1250.001941197816.5603185
GTATAAT7550.016.4506471
CGATCTG77700.016.2512785
Produced by FastQC (version 0.11.2)