FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021221
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9350.020.2940545
CGATCTT76150.019.571835
GCCGTCT9050.019.44192546
TATGCCG8900.019.3819643
CGATCTG130050.018.7288885
ATGCCGT10300.017.41741844
CCGTCTT10250.017.16579647
CCGATCT343000.016.9280784
CGTATGC10500.016.75725441
AGACCGC6800.016.74259269
GATCTTT57850.016.6983016
TTCCGAT359850.016.1929442
TCCGATC365650.016.011573
CTTCCGA372400.015.74931
GTATATA36550.015.1970911
GATCTGG50550.014.4005456
ATAATAC22400.014.3236123
GTATTAG16650.014.0901831
ATCTGCG15050.013.9833427
GTATTAT21250.013.8000921
Produced by FastQC (version 0.11.2)