FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17748442
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA199730.11253382127850997No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG190470.1073164619181785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA71350.021.80720315
CCGGTAA74150.020.98373416
CGGTCCA81100.019.61095210
ACGGTCC82750.018.8446929
GCGTCGT84800.018.38907832
TCGCGTC84700.018.37005830
CGCGTCG86850.018.11391831
AATACGC87000.018.00337821
ACGCTTA86350.017.97908424
TAATACG87250.017.8727120
TACGCTT88300.017.77739523
GATCTGG186300.017.4629926
GACGGTC90900.017.3828188
TCCACCG91900.017.34383212
CTCGCGT91000.017.28784629
GTCGTAA144950.017.13692534
CCGATCT1328150.017.0220434
GTCTTAC121500.016.9802281
CGCTTAC92500.016.93290725
CGATCTG589400.016.887075
Produced by FastQC (version 0.11.2)