FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337981
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3400.031.45566745
ATGCCGT3950.029.69644
TATGCCG4750.024.69456943
GCCGTCT4150.024.1082746
TCGTATG4800.023.71909540
CGATCTT44150.020.4736825
CGTATGC6050.019.38871241
CGCCGTT8550.019.36828825
TTCGATC7150.019.3009841
TCTGTCG4750.018.8844878
GGACCGT10600.018.8776346
TACGCTA6950.018.86358560
TCGATCA7050.018.59561742
CGCTAAT1501.695238E-518.39987415
CGATCAA7350.018.30599843
CGCTAAC8300.017.87375562
GACCGTT10650.017.8171627
GCCGTTT9550.017.7014526
ACCGTTA9950.017.6836768
GCTTCGC8050.017.57130852
Produced by FastQC (version 0.11.2)