FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897765
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC750.001993564422.9998687
GACGGCG1004.3356346E-420.70042662
TATGCCG1901.6003469E-819.9735743
TACGTCG1056.025203E-419.7141747
AGACCGC2800.019.71417269
CGATCTT31850.019.1725735
TGCCGTC1852.6454472E-718.6485445
CGCGTCC1753.2101998E-617.74275657
TCTGCGC3650.017.0136018
GATCTTT26200.016.459836
CGATCTG62000.016.192655
ATCTGCG5750.015.599917
GTATAGA6500.015.392221
CTATACG1808.090197E-515.3332464
CGGTCGG2705.2536052E-815.33324468
ATGCCGT2302.5757963E-614.99991544
CCGATCT166150.014.701094
CGTATGC2353.2195167E-614.68115441
TAGACGT8700.014.672334
GTATAAG5900.014.618561
Produced by FastQC (version 0.11.2)