FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78215
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG26773.4226171450489034TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG16242.076328070063287TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG7991.0215431822540433TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG4840.6188071341814231TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG3940.50373969187496TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG2930.37460845106437385RNA PCR Primer, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG2230.2851115514926804RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG2000.25570542734769547TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG1700.2173496132455411TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGGGGG1700.2173496132455411TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG1490.1905005433740331TruSeq Adapter, Index 10 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1430.18282938055360226TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGGGGG1350.17260116345969445TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1320.168765582049479Illumina PCR Primer Index 5 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGG1300.16620852777600204TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGGGG1070.13680240363101706TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1010.1291312408105862Illumina PCR Primer Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAGGGGG930.11890302371667838TruSeq Adapter, Index 10 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG880.11251038803298599RNA PCR Primer, Index 10 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG880.11251038803298599TruSeq Adapter, Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG870.11123186089624752TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG850.10867480662277056Illumina PCR Primer Index 5 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG850.10867480662277056TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGTGGGG840.10739627948603209TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG830.10611775234929362Illumina PCR Primer Index 5 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG810.10356069807581667RNA PCR Primer, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG810.10356069807581667TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG150.002347326869.053
TAAAAGG150.002347326869.067
CCAAGTA150.002347326869.052
ACAGGGG353.2474236E-759.14285769
TCGTTTG252.5523733E-455.19999740
GTGCTTG1000.055.19999754
GTCTTGT1200.054.62549
CTTGTGC1100.053.3181851
TCTTGTG1250.052.4450
CATATAC408.165989E-751.751
AGGAACA200.007333553451.7526
AGTCTTC200.007333553451.7548
CTCGTTT352.2533266E-549.28571739
TGTGCTT1200.048.87553
TGCTTGT506.834307E-848.29999555
CGTCTTG1450.047.58620548
GTTCTGC306.275262E-446.051
GCAGTCT306.275262E-446.046
GCTTGTA605.496986E-946.056
ATATACA451.8390874E-646.02
Produced by FastQC (version 0.11.2)