HW3HFAFXX_n01_zt14ss56.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW3HFAFXX_n01_zt14ss56.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94137
Sequences flagged as poor quality	0
Sequence length	75
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	967	1.0272262765968747	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG	601	0.6384312225798571	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG	175	0.18589927446168883	RNA PCR Primer, Index 39 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG	127	0.13490975918076845	RNA PCR Primer, Index 39 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG	112	0.11897553565548084	Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	95	0.10091674899348822	RNA PCR Primer, Index 39 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAC	15	0.0023488158	69.0	7
TGTATGC	15	0.0023488158	69.0	19
ATACTTA	15	0.0023488158	69.0	6
TGCGATA	15	0.0023488158	69.0	23
ATGTTGT	20	8.473701E-5	69.0	32
TGCTTGA	615	0.0	57.21951	55
CTCGTAT	715	0.0	56.454544	39
CTTGAAA	695	0.0	56.093525	57
TCTCGTA	665	0.0	56.030075	38
CCGTCTT	685	0.0	55.905113	47
TACATCT	685	0.0	55.905113	34
CATCTCG	700	0.0	55.692856	36
TCGTATG	775	0.0	55.645157	40
ACGGGGG	50	1.0968506E-9	55.2	69
TTGAAAA	750	0.0	55.2	58
ATCTCGT	685	0.0	54.89781	37
GTCTTCT	610	0.0	54.860653	49
CGTATGC	805	0.0	54.85714	41
GTATGCC	805	0.0	54.85714	42
GCTTGAA	700	0.0	54.70714	56