FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65761
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG12231.8597649062514254RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG6480.9853864752664954RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG1870.28436307233770775RNA PCR Primer, Index 39 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1810.2752391234926476Illumina PCR Primer Index 11 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1370.2083301652955399RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG1090.16575173735192592RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG960.14598318152096226RNA PCR Primer, Index 39 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG930.1414212070984322RNA PCR Primer, Index 39 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG900.13685923267590214Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG670.10188409543650492RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTC150.00234586368.9984810
CAAGGTA150.00234586368.9984816
GGACGTG150.00234586368.998486
AGTTACG150.00234586368.9984824
AATGTAG150.00234586368.998485
CTACAGC501.6370905E-1162.0986333
GCCGTCT6450.057.7661746
CCGTCTT6550.057.41094647
TCTGCTT5650.056.17575553
GTATGCC7400.055.9447142
TCGTATG7050.055.78600340
TATGCCG7550.055.74711243
CTGCTTG5700.055.68298754
CGTATGC7250.055.67463741
CTTGAAA6350.055.416157
TGCCGTC7100.055.39314745
GTCTTCT5800.055.3177449
TTGTGGT252.5496486E-455.1987852
AGGGGGG1750.055.1987869
TGCTTGA6100.054.85944455
Produced by FastQC (version 0.11.2)