FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411198
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3900.028.30763244
TGCCGTC3600.027.79160745
TCGTATG3750.026.68049840
GCCGTCT4100.025.2438546
CGTATGC4050.024.70365141
CTCGTAT4950.020.90948139
CCGTCTT5650.020.150447
CCGTACG1056.0257065E-419.7142459
CGATCTT41550.018.9313685
CCGTTTA3150.018.61900927
CGATCTG85900.017.7118365
GCTATCG2359.495125E-917.61698313
CGTTTAC3400.017.24996428
GATCTTT31150.017.0561456
GTATGCC6500.016.9845842
GATCTGG29250.016.7486846
GGCGACG5650.016.4866965
TCTGTCG5750.016.1999668
CTGTCGC5150.016.0776379
CGCGGTT7400.015.85131820
Produced by FastQC (version 0.11.2)