FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10421423
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12800.027.22257643
CGATCTT236300.025.6815955
TGCCGTC14250.022.9999345
GATCTTT231650.022.8015756
CGATCTG192000.021.9398845
ATGCCGT16550.021.47123544
CCGATCT648950.021.4405984
TTCCGAT667050.020.858822
TCCGATC682200.020.4967383
CGTATGC17400.020.42254841
ATCTTTT263550.019.6619597
TCGTATG18950.019.48034140
CCGTCTT16350.019.20177747
CTTCCGA745950.018.7635571
GCCGTCT16350.018.5687546
AATCGCG1753.2129228E-617.74280468
CTCGTAT20450.017.03923239
GATCTGG90050.016.8191016
ATCTCGC22900.016.572087
ATCTGCG22300.016.3991327
Produced by FastQC (version 0.11.2)