FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055552
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2800.030.80424347
TATGCCG3300.028.22789243
TGCCGTC3300.028.22789245
GCCGTCT3100.026.7102646
ATGCCGT3550.026.24000744
CGATCTT34350.023.401695
CGTATGC4000.023.28744341
TCGTATG4100.022.71945640
CTCGTAT4150.020.78308139
TACATCG1356.8277914E-620.44439369
GATCTTT32450.020.0939436
TGTACCG1056.0253066E-419.7142375
AATACCG1056.0253066E-419.7142375
CGATCTG38450.019.2015135
CGTCTTC4350.018.2417848
CCGATCT121300.017.7192484
TTCCGAT120650.017.6717342
TCCGATC127800.016.8180353
GTATGCC5400.016.61106942
ATCTTTT39300.016.5037777
Produced by FastQC (version 0.11.2)