FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899368
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3450.028.99973343
GCGCGTA605.4238056E-428.74973720
CGCGTAA605.4238056E-428.74973721
GCCGTCT2850.027.8418546
ATGCCGT3500.027.59974744
TGCCGTC3200.026.95287945
CGATCTT60200.023.0379965
CGATCTG42400.023.0269135
CCTCGTT1052.2819562E-522.9997933
CCGTCTT3600.022.04146647
CCGATCT145800.021.9349844
TTCCGAT148850.021.6483382
GATCTGG17750.021.574456
CGACGTC800.00289983221.5628766
GATCTTT58800.021.2982756
TCGTATG5200.021.23169340
CGTATGC4950.020.9094541
TCCGATC154550.020.827053
TAGGGGT5650.020.7608724
CTCGTAT5300.020.18012439
Produced by FastQC (version 0.11.2)