FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361037
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA405.0025104E-543.12500463
TATGCCG1650.029.27272643
ATGCCGT1902.7284841E-1123.60526344
TCGTATG2200.023.52272840
CGTATGC2103.6379788E-1223.00000241
CGATCTT20700.022.1666665
TGCCGTC2057.2759576E-1121.8780545
GACGCCG800.002899702921.56250254
CGACGTC1004.3353703E-420.766
CTCGTAT2655.456968E-1219.52830139
GAACGAG900.00573494819.16666664
GTATAAG3600.019.1666661
GACCGCT1108.237785E-418.81818260
TCTCGTA2651.1641532E-1018.22641638
ATCTGCG3103.6379788E-1217.8064527
TATACTT5150.017.4174775
GATCTTT17100.017.3508786
CGCGGGA1200.001476966817.2513
CGAACTT1402.0829332E-417.24999833
TTATACT5650.017.0973454
Produced by FastQC (version 0.11.2)