FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536525
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGG47080.3064056881599714No Hit
GATTAATAGGGATAGTCGGGGGCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACT43710.28447308049006687No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACAT41220.26826768194464784No Hit
ATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCC35030.22798197230764222No Hit
TTATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATC30800.2004523193569906No Hit
GAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGGGCGTCA27570.17943085859325425No Hit
CTATTATTCCATGCTAATGTATTCGAGCAAAGGCCTGCTTTGAACACTCTAATTTTTTCACAGTAAAAGTCCTGG23740.15450448251736873No Hit
GTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTGCGAAAGCATTCGCCAAGGATGTT23720.15437431867363044No Hit
CTCTACTTGTGCGCTATCGGTCTCCGGCCAATATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATT22810.14845186378353753No Hit
GGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACATCCTTGG22570.14688989765867785No Hit
GTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACATCCTTGGCGAATGCTTTCGCAGT22500.1464343242055938No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCGCGGACACCCCATCCCATACG22280.14500252192447244No Hit
GGTCATTATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTT21870.14233416312783717No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACG18200.11844909780185808No Hit
GTGCCGAACGGGTCATTATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATC17070.11109484063064382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG8600.034.57
TGCGCTA8900.033.3370810
GCTATCG9050.032.02210213
GCGCTAT9400.031.5638311
CGCTATC9250.031.3297312
ACTTGTG9650.030.7461135
GCGGGTT450.004623170530.66666614
TGTGCGC10100.030.0594068
TACTTGT10250.029.6195134
GTGCGCT10150.029.5714289
TCCGGCC9900.028.22727223
TATCGGT10300.028.13592115
CTACTTG10550.028.1232223
TACGGAC1850.027.97297113
CGATCTT17300.027.121395
CTTGTGC11200.026.491076
CGGCCAA10850.025.7557625
CGGTCTC11400.025.42105318
CTCCGGC11150.025.0627822
TGGTACG2100.024.64285910
Produced by FastQC (version 0.11.2)