FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67815
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG7721.138391211383912TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG5230.7712158077121581TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG1690.24920740249207404TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1170.17252820172528202RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG1090.160731401607314TruSeq Adapter, Index 1 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1090.160731401607314TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG940.13861240138612402RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCA150.00234597569.013
GTCCGCT150.00234597569.038
TCACTCC150.00234597569.09
CACAAGC150.00234597569.019
AGCTACA150.00234597569.056
GCGTATG309.034149E-657.540
TGGTTGA252.549941E-455.19999755
GGTTGAA252.549941E-455.19999756
GCCTTAG200.00732936451.7551
TCACCAG200.00732936451.7545
GATAACT200.00732936451.7537
TCCTGAG200.00732936451.7523
TAAACTC200.00732936451.7527
AGGTTTA200.00732936451.7550
ATATACC200.00732936451.754
CCTCAAC200.00732936451.7547
CCAGTCA200.00732936451.7531
GTCGTAT552.5356712E-950.1818239
TATATAC352.25012E-549.2857173
GTCTTCT5400.048.55555349
Produced by FastQC (version 0.11.2)