FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56258
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10771.9143943972412814TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG6561.1660563830921824TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG1790.31817697038643394TruSeq Adapter, Index 1 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1520.27018379608233495TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1120.19908279711329946RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG1060.1884176472679441TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG1030.18308507234526644TruSeq Adapter, Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG1030.18308507234526644RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG800.14220199793807103TruSeq Adapter, Index 1 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG780.13864694798961927TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGGGGG600.10665149845355328TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG150.0023438969.06
GCAAGTG150.0023438969.045
CGTATGT208.4441206E-569.030
CATATAC150.0023438969.01
GGTCCCC208.4441206E-569.068
GAGAGAG150.0023438969.08
TCTAGGC150.0023438969.067
GAAACAC150.0023438969.022
CTGTTTT150.0023438969.030
CGGTCCC208.4441206E-569.067
TGGGCCC208.4441206E-569.051
AGAGAGC150.0023438969.09
AAACCTA253.0563187E-668.9999959
CCTACCG252.546189E-455.19999762
ACCTACC252.546189E-455.19999761
TTTGCAA252.546189E-455.19999742
TAGCTCG252.546189E-455.19999725
GGAATTT200.007322898651.7538
GCTTCTA200.007322898651.7564
ATATACA200.007322898651.752
Produced by FastQC (version 0.11.2)