FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228141
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT2650.022.1319186
GACCGTT2950.021.0506977
ACCGTTA3000.020.6998528
TCTGTCG4700.020.5530438
CCGTTAT3250.019.1075559
CGATCTG66750.018.8133495
ATGCCGT3550.018.46465744
TATGCCG3200.018.32799343
GTATTAA7700.017.921951
TTTCGGA2902.1827873E-1117.84471
AGGACCG3700.017.716095
CCGCCGT3900.017.6921824
CGATCTT35250.017.6168965
TACGGCC3350.017.50733819
CTGTCGC5200.017.2498789
ATACCGC2601.7716957E-917.2498786
CGCCGTT3600.017.24987625
TGCCGTC3650.017.01357845
CGTATGC3501.8189894E-1216.75739941
GATCTGG24300.016.6109926
Produced by FastQC (version 0.11.2)