FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578059
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT802.7625774E-630.18740565
ACCGTAC500.00773589927.5999138
TATGCCG2151.4006218E-1020.86039743
GCCGTCT2201.9099389E-1020.38629746
CGACCCG850.004120173420.29469537
CGATCTG41950.019.9844425
TGCGCGG1409.347257E-619.71422210
ACCGTTA1409.347257E-619.7142228
GGACCGT2102.3519533E-919.7142226
CGTATGC2354.6020432E-1019.08504541
GATCTGG14850.018.3534766
CCGTCTT2458.021743E-1018.30606547
AGGACCG3153.6379788E-1217.5237525
TCTGCGC2601.7698767E-917.2499458
CGATCTT21250.017.0470055
TCGTATG2702.921297E-916.61158440
TGCCGTC2702.923116E-916.61105745
CCGATCT112800.016.485324
TGTCGCC2955.366019E-1016.37282810
TTCCGAT114900.016.3041252
Produced by FastQC (version 0.11.2)