FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663612
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGC2500.022.0805959
CGTATGC2151.4006218E-1020.86040341
GTAAGCG850.004121018620.2940543
GTCCGGT1258.7442255E-519.3199460
GCCGTCT2505.0931703E-1119.3199446
TATGCCG2354.6020432E-1019.08504743
CGATCTG46150.018.9880245
CGAAATA1108.239008E-418.81812558
TACACCG1301.1814574E-418.5768665
ACGTCCG950.00783602318.1578458
TCCGCAC1351.5775108E-417.88883451
CCGTCGA1351.5775108E-417.88883458
CGTCGAG1351.5775108E-417.88883459
CGTCCGG1552.2470767E-517.80639659
TGTCGCC2551.366061E-917.5887110
TCGTATG2551.36788E-917.5881840
TCTGTCG3400.017.2504658
CGATCTT27250.017.091695
TGCCGTC2552.600791E-816.23524545
GGCGACG3209.822543E-1116.17182565
Produced by FastQC (version 0.11.2)