FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027816
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1800.028.7541
TATGCCG1700.028.41176443
TGCCGTC1601.8189894E-1228.03125245
TCGTATG1900.027.23684340
GCCGTCT1759.094947E-1225.62857246
ATGCCGT2103.6379788E-1222.99999844
CCGTCTT2151.4006218E-1020.86046647
ATCGGAT1751.4723628E-719.71428541
CGATCTT16150.019.4396295
GTATAAA5100.018.9411751
GGGGTGC5150.018.7572826
TGCACCG1150.001110114418.00000210
CCGAAAA4850.017.78350417
ACTCGTC2359.476935E-917.61702256
CGGATGA2551.366061E-917.58823643
GTATGCC2952.910383E-1117.54237242
CGATCTG29200.017.368155
CCGTGCA1200.001476634117.259
TCTCGTA2601.7680577E-917.2538
CGGGCGA1402.0823076E-417.2563
Produced by FastQC (version 0.11.2)