FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735883
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGA850.00412113520.29401855
GCGACGG1952.156412E-819.46144166
CGCAACG950.00783624918.15780362
ACTGTCG1552.2472062E-517.8063628
TGTCGCC2904.2018655E-1016.65508810
ATAATAC6250.016.5603943
TATGCCG2301.4240322E-716.49991843
CGATCTG57000.016.4030235
GGACCGT1907.0234673E-616.3420226
GATCTGG19350.016.2247266
ATGCCGT2351.8189166E-716.14885544
GCCGTCT2351.8189166E-716.14885546
CGATCTT24500.016.0534445
CGTAGGG3058.60382E-1015.8364422
GTATTAT6550.015.8019031
TAGACGT7050.015.6599474
ACCGTAC1350.003244041715.3332578
TGCGTTG1350.003244041715.33325710
GGCGACG3857.2759576E-1215.2336965
GCGGGTA1605.760385E-415.09367414
Produced by FastQC (version 0.11.2)