FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491449
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12200.026.01629343
TCGTATG13050.024.32217640
GCCGTCT13350.023.51676446
TGCCGTC13950.022.01066845
CGTATGC14850.021.60623241
CCGTCTT15150.021.17813747
ATGCCGT15200.020.65452644
CGATCTT145900.019.1534565
GCGACGG10100.018.44558366
GATCTTT112550.017.1045346
GGCGACG22450.016.44324165
CTCGTAT19100.015.534245539
CGATCTG290900.015.2871525
CGTAATA4400.014.8977582
AGGGCGA18050.014.52634563
CCGATCT757800.014.3499054
GATCTGG87550.014.3439176
TTATACT38350.014.3037264
GCGACGT11600.014.2758966
GTATGCC22750.014.2548942
Produced by FastQC (version 0.11.2)