FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77577
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG6170.7953388246516365RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG2550.3287056730732047RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG1070.13792747850522705RNA PCR Primer, Index 27 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1020.13148226922928188RNA PCR Primer, Index 27 (100% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG930.11988089253258054RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT208.464312E-569.04
GATACAT150.002347252869.033
ATGTCGT150.002347252869.037
GGGGTGT150.002347252869.055
CCTTTCG150.002347252869.030
TCGCTAA150.002347252869.046
TGTAGTT150.002347252869.039
CGCACCA150.002347252869.063
CGATACA150.002347252869.032
GGTGTAA253.0672854E-668.9999957
ATAGGGG252.5522406E-455.19999768
ATAGAGT252.5522406E-455.1999974
ATACTGA252.5522406E-455.1999976
GTTTGCC700.054.21428742
CTATCGC1050.052.57142635
TATGCCG5650.051.90265343
GCCTTCT200.00733332351.75000455
CATATTG200.00733332351.75000416
GAGTCAA200.00733332351.75000464
TCCGAAC200.00733332351.7500043
Produced by FastQC (version 0.11.2)