FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676796
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG46200.029.1994275
CCGATCT117150.026.652984
TTCCGAT118650.026.2283082
TCCGATC122350.025.6325233
CGATCTT32450.024.9857675
CTTCCGA132250.023.687631
CTCGACG1803.43789E-1022.99979616
TACGCGA1202.4600595E-622.99979429
GTACGCG1202.4600595E-622.99979428
ATCTGCG5150.022.7773467
TCGACGA1854.838512E-1022.37817817
CGTTTCG800.00289932221.5639176
GATCTGG24300.021.4396826
GCGAATG5200.021.2305869
CGATCTA15400.020.8353985
GAACCGT1004.3349527E-420.7013636
TCTGCGC2501.8189894E-1220.7005888
ACTCGGC2201.9099389E-1020.38656235
GAATCGG2201.9099389E-1020.3861869
TAGGACG850.004120467720.2950734
Produced by FastQC (version 0.11.2)