HW3HFAFXX_n01_zt14ss39.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW3HFAFXX_n01_zt14ss39.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75151
Sequences flagged as poor quality	0
Sequence length	75
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	675	0.8981916408297961	RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG	266	0.3539540392010752	RNA PCR Primer, Index 27 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG	92	0.12242019400939441	RNA PCR Primer, Index 27 (100% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG	89	0.11842823116126198	RNA PCR Primer, Index 27 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAC	15	0.002346969	69.0	22
TATTCCG	20	8.46261E-5	69.0	43
AAATTAC	15	0.002346969	69.0	27
GCACTCG	15	0.002346969	69.0	4
TCAGTAA	15	0.002346969	69.0	2
GAGGCAC	15	0.002346969	69.0	1
CTCGTTT	55	0.0	62.72727	39
TCTCGTT	60	1.8189894E-12	57.5	38
GTAAAAA	60	1.8189894E-12	57.5	60
TCGTTTG	55	4.0017767E-11	56.454544	40
GTATTAC	25	2.5517296E-4	55.199997	40
GTTTCAG	25	2.5517296E-4	55.199997	8
GGTGTAA	20	0.007332445	51.75	57
AGGAGCG	20	0.007332445	51.75	62
TGGGTGT	20	0.007332445	51.75	55
TATGCCC	20	0.007332445	51.75	43
AGTATTA	20	0.007332445	51.75	39
AGACGTC	20	0.007332445	51.75	54
CACTCGG	20	0.007332445	51.75	5
GTATGAA	20	0.007332445	51.75	62