FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439583
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2600.026.53840443
TCGTATG3100.021.14511740
CGTATGC3500.020.69995741
CCGTCTT3350.019.56712347
AGACGGC5350.019.34575569
TATCGCG950.00783718.15785836
TGCCGTC3650.017.95886645
CGTTCGA1351.57788E-417.88885310
ACCGTAC1753.2102216E-617.7431858
CGATCTG82700.017.6466375
CGATCTT43850.017.6240435
GCCGTCT3950.017.46831946
ATGCCGT4150.017.45779644
ACGGACG1653.8445098E-516.72723869
GATCTGG27550.016.279796
GACCGTA2151.2763594E-616.0468067
TTTCGGA3900.015.9230441
CCGATCT223800.015.5391614
CCTATCG2907.539711E-915.46548569
CGAACAT1808.09146E-515.33330153
Produced by FastQC (version 0.11.2)