FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157011
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT4500.032.19999747
GCCGTCT4200.032.03571346
AGACGGC8600.031.29069969
ATGCCGT5300.030.5943444
TGCCGTC5500.027.645
ATCTGCG7350.026.2857157
CGATCTG61350.026.2616125
GACTCCG5300.024.08490669
CGATCTT84600.024.0195055
TTTCGGA9200.024.01
GATCTTT73750.023.5301696
TCGTTTG1202.4594992E-623.040
TATGCCG6900.023.043
CCGATCT223000.022.9587444
GATCTGG24000.022.281256
TATCTCG6850.022.16058336
TTCCGAT231250.022.1396772
TTCGGAA9400.022.0212782
CGTCTTC5800.022.00862148
TCGTATG6800.021.81617540
Produced by FastQC (version 0.11.2)