FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030620
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2402.7284841E-1120.12543
TTTCGGA2759.094947E-1218.8181821
AGACGGC5200.017.91346269
GTATTAG5750.017.41
CGATCTT38300.017.295045
AGCGTTC1200.001477305417.258
AGGGCGA3600.017.2563
TATACTG9000.016.8666675
CGTATGC3157.6397555E-1116.42857241
TCTGGCG2955.366019E-1016.372888
CGATCTG71800.016.2409485
ATGCCGT3006.8030204E-1016.144
GATCTTT24850.015.82696156
ACGCGTC1756.3604515E-515.77142956
GATCTGG24500.015.6306116
GGACGGT1554.526246E-415.58064559
GTATTAT6700.015.4477621
GTATATA13200.015.1590911
ATCTGCG5250.015.1142857
TCTGTCG5500.015.0545458
Produced by FastQC (version 0.11.2)